There is no question that social media is changing the way we communicate with each other. For professionals, LinkedIn® is one of the most popular platforms today, allowing us to connect with colleagues, share information and stay up-to-date on the latest industry trends and topics. That’s why we are moving this ChromaTALK platform to LinkedIn

Once a target protein has been identified to become a potential drug, the purification process has to yield an even higher quantity of proteins for further studies. While it is critical to maintain the product’s purity and to fulfill all requirements in terms of quality, the purification processes have to be scaled up – usually from the mg-level up to ...

Based on recent advances in biotechnology, the introduction of protein and peptide drugs is growing rapidly. Nearly all applications require a high purity. Thus, efficient chromatographic column separations are necessary. The most important technique is ion exchange chromatography (IEX). This technique results in high purities combined with high recoveries while the operational costs are rather moderate. In addition, for ion ...

Biologicals like monoclonal antibodies carry with them a risk of viral contamination. This risk is generally mitigated through careful screening of source materials and raw materials and monitoring of process intermediates for the presence of viruses. However, it is possible that viruses may enter the process and escape detection. For this, viral clearance during ...

What we are seeing as we work with customers is a drive to simplify and compress processes as much as possible.   Perhaps five years ago, there was a protein A column, low pH step, cation exchange (CEX), anion exchange and a virus filtration step.   What we see now is customers who don’t include, or ...

For recombinant protein and monoclonal antibody produced on mammalian cell line like CHO cells, virus inactivation is frequently accomplished through low pH treatment and detergent treatment at low concentration.  Both steps are suitable to inactivate a specific group of virus which are envelope viruses, but are not capable of inactivating the more resistant,  non-envelope ...

There are well-established processes for virus clearance from recombinant proteins in downstream processes including inactivation by low pH, anion and cation exchange chromatography and virus retention filtration.  The typical approach is to calculate the log retention values across each step and sum up at the end for the entire process.  If one is using ...