Topic #3: What’s in Your Column: Irregular or Spherical?

For years, a robust debate about the merits of irregularly and spherically shaped column packings took place. While the dust has somewhat settled to a great extent, tell us what you think. Is spherical the preferred material of choice due to higher efficiency and better quality of separations? What about cost? Can you get equivalent efficiency with irregular material? Do you evaluate both during process development?

Our experts discuss…

Prof. Klaus K. Unger The treatment of mass transport phenomena of HPLC between 1960 and 1970 predicted that one needs small particles of porous silica, say five to ten microns to achieve a high column efficiency, and these particles were originally made by grinding and milling larger particles of silica. You lost a…

Dr. Michael SchulteWhen the whole history of silica chromatography started, the first high-performance materials, even for larger scale separations, were all irregular. One of the main arguments for the use of spherical materials was always that they would offer higher efficiency.

A spherical material can withstand a higher pressure and thus packing more…